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5th Symposium on Structural Proteomics

November 19-20, 2015
Halle (Saale), Germany
Cross-linking
HDX
Covalent modification
Limited proteolysis
Ion mobility Native MS
Affinity labeling
Protein structure modeling
Organizing committee:   www.cost.eu
Andrea Sinz
Evgeniy Petrotchenko
Christoph Borchers
Confirmed speakers:
Perdita Barran, Manchester, UK
Julia Chamot-Rooke, Paris, France
Frank Herzog, Munich, Germany
Olaf Jahn, Goettingen, Germany
Petr Novak, Prague, Czech Republic
Kevin Pagel, Berlin, Germany
Michal Sharon, Rehovot, Israel
Program:
Thursday, November 19, 2015
8:30 -9:00                          Registration
9:00 – 9:15                         Welcome; Michael Bron, Vice Principal Martin Luther University Halle-Wittenberg; Andrea Sinz, local organizer
9:15 – 10:00                       T1: Michal Sharon(Weizmann Institute, Rehovot),Discovering a new subunit for an old complex by native mass spectrometry
10:00 – 10:30                     T2: Petr Novak(Academy of Sciences, Prague), When X-ray crystallography fails: CD302 Antigen Structure via Mass Spectrometry and NMR spectroscopy
10:30 – 11:00                    Coffee Break
11:00 –11:15                      T3: Charlotte Uetrecht (Leibniz Inst. Exp.Virol., Hamburg), Studying viral assemblies with mass spectrometry
11:15 – 11:30                     T4: Carla Schmidt (University of Oxford),Dimeric 13-Subunit Cytochrome C Oxidase is Stabilised by Lipids and Regulated by Post-translational Modifications
11:30 – 11:45                     T5: Janne Jänis (University of Eastern Finland), Ligand Binding Preference of a Macrolide Specific Repressor Protein MphR(E)
11:45 – 12:00                     T6: Nina Morgner (Frankfurt University), LILBID-MS on membrane protein complexes
12:00 – 12:15                     T7: Knut Kölbel (University of Antwerp), Conformational Properties of Gas Phase Protein Molecular Ions from a Thermodynamic Perspective
12:15 – 12:30                     T8: Marc Kipping (Waters), Extensive Charge Reduction and Dissociation of Intact Protein Complexes Following Electron Transfer on a Quadrupole-Ion Mobility-Time-of-Flight MS
12:30 – 12:35                     PT1: Dietmar Hammerschmid (University of Antwerp), Structural characterization of the globin coupled sensor from Geobactersulfurreducens (GsGCS) by using native mass spectrometry
12:35 – 12:40                     PT2: Jingzhi Yang (Proteome Center Rostock), From antibody structure - function correlation to multiplexed immuno-assays
12:40 – 12:45                     PT3: Albert Konijnenberg (University of Antwerp), Extending structural proteomics to integral membrane proteins
12:45 – 14:30                    Lunch and Poster Session
14:30 – 15:10                     T9: Franz Herzog (LMU Munich),Cross-Linking and Mass Spectrometry: Architecture and Mechanism of Chromatin-Associated Complexes
15:10 – 15:40                     T10: Olaf Jahn (MPI Exp. Med. Göttingen), Structural characterization of synaptic calmodulin complexes by photoaffinity labeling and mass spectrometry: the (everlasting) Munc13 story
15:40 – 16:00                    Coffee Break
16:00 – 16:15                     T11: Nir Kalisman (Hebrew University of Jerusalem), Two years later – The XL-MS model of the transcription pre-initiation complex revisited
16:15 – 16:30                     T12: Alexander Leitner (ETH Zurich), Monitoring large conformational changes in protein complexes with a dedicated quantitative cross-linking software pipeline
16:30 – 16:45                     T13: Jens Meiler (Vanderbilt University), De novo protein structure prediction from crosslinking data
16:45 – 16:50                     PT4: Valentina D’Atri (Univ. Bordeaux, INSERM), Structural characterisation of human telomeric G-quadruplexes by using Ion Mobility Mass Spectrometry (IM-MS) and Molecular Dynamics (MD) simulations
16:50 – 16:55                     PT5: Pavel Reichmann (IPB Halle), Description of an N-End Rule E3 Ligase Substrate Binding Site
16:55 – 17:00                     PT6: Verena Tinnefeld (ISAS Dortmund), Enrichment of cross-linked peptides from complex samples by charge-based fractional diagonal chromatography
17:45 – 18:45                    City Night Tour through Halle (meet at Händel statue)
19:00 – Open end            Dinner Palais S (Address:  Ankerstraße 3c, 06108 Halle) with Live Music (Schema F)
 
Friday, November 20, 2015
9:00 – 9:40                         T14: Perdita Barran (The University of Manchester), Adventures with Disorder and Charge
9:40 – 10:10                       T15: Julia Chamot-Rooke (Institut Pasteur, Paris), Application of top-down mass spectrometry to microbial proteomics
10:10 – 10:40                    Coffee Break
10:40 – 10:55                     T16: Philip Lössl (University of Utrecht), Integrating native (ion mobility) MS, cross-linking/MS and top-down proteomics to decipher the interplay between phosphorylation and interactions of the mitotic regulators Aurora A, Bora, and Plk1
10:55 – 11:10                     T17: StéphanieDeroo (University of Brussels), Towards the receptor-active conformation of apolipoprotein E: a combined XL/MS and molecular dynamics study
11:10 – 11:25                     T18: Nadine Harrer (LMU Munich), The structural architecture of the chromatin remodeler ISWI determined by cross-linking and mass spectrometry
11:25 – 11:40                     T19: Giambattista Guaitoli (DZNE Tübingen), Crosslinking-MS analysis combined with molecular modelling provides novel insight into the domain architecture of the PD-associated protein kinase LRRK2
11:40 – 11:55                     T20: Gert von Helden (Fritz Haber Institute Berlin), Unzipping of gas phase proteins - similar secondarystructures at high charged states
11:55 – 12:10                     T21: Tam Nguyen (University of Copenhagen), A novel CE-MS interface for top-down MS analysis of proteins
12:10 – 12:15                     PT7: Waldemar Hoffmann (Fritz Haber Institute Berlin), Gas-Phase Infrared Spectroscopy of Ion Mobility-Selected Amyloid Intermediates
12:15 – 12:20                     PT8: Melanie Göth (Free University Berlin),Gas-Phase Microsolvation of Ubiquitin: Identification of Crown Ether Complexation Sites
12:20 – 12:25                     PT9: Patrick Knight (University of Leeds), Characterising the interaction of ataxin-3 and the poly-glutamine aggregation inhibitor QBP1
12:25 – 14:30                     Lunch and Poster Session
14:30 – 15:00                     T22: Kevin Pagel (Free University Berlin), Separation of Oligosaccharide and Glycopeptide Isomers using Ion Mobility.Mass Spectrometry
15:00 – 15:15                     T23: Mathias Müller (Thermo Fisher Scientific), Analysis of Native Protein Complexes on a Benchtop Orbitrap Mass Spectrometer
15:15 – 15:30                     T24: Dana Reichmann (Hebrew University of Jerusalem), The nature and role of stress-regulated protein disorder in cellular proteostasis
15:30 – 15:45                     T25: Pernille Foged Jensen (University of Copenhagen), Dissecting the FcRn binding mode of antibodies with different pharmacokinetic profiles by hydrogen/deuterium exchange mass spectrometry
15:45 – 16:00                     T26: AlesHnizda (Academy of Sciences, Prague),Integrative structural biology of relapsed-ALL specific cN-II mutants
16:00 – 16:05                    PT10: Rasmus Jakobsen (University of Copenhagen), Implementation of online electrochemical reduction of proteins into a Hydrogen/ Deuterium exchange Mass Spectrometry workflow
16:05 – 16:10                    PT11: Patrick Merkle (University of Copenhagen) Studying the Interaction between Affinity Enhanced T-Cell Receptors and Peptide-Bound Major Histocompatibility Complexes by Hydrogen/Deuterium Exchange Mass Spectrometry
16:10 – 16:15                    PT12: Marcus Nagel (UFZ Leipzig), First full-length Thyroid stimulating Hormone-Receptor structure elucidated by low resolution techniques
16:15 – 16:20                    PT13: ZsuzsannaOrban-Nemeth (IMP Vienna), ComprehensiveXL-MS reveals parallel orientation and versatile conformations of plant HOP2/MND1
16:20 – 16:25                    PT14: Gabriel Mazzucchelli (University of Liege), De novo sequencing using MELD proteolysis coupled to "sequence assembly" algorithm
16:25 – 16:30                    PT15: Michal Rosulek (Charles University Prague), From monomer to tetramer: Mass spectrometric study of protein WrbAmultimerization
16:30                                  Goodbye
16:35                                 Farewell Coffee
17:00                                End of Symposium
 
 
Poster Presenters:
Dietmar Hammerschmid Structural characterization of the globin coupled sensor from Geobacter sulfurreducens (GsGCS) by using native mass spectrometry 
Jingzhi Yang From antibody structure - function correlation  to multiplexed immuno-assays 
Albert Konijnenberg Extending structural proteomics to integral membrane proteins 
Valentina D’Atri Structural characterisation of human telomeric G-quadruplexes by using Ion Mobility Mass Spectrometry (IM-MS) and Molecular Dynamics (MD) simulations
Pavel Reichman Description of an N-End Rule E3 Ligase Substrate Binding Site 
Verena Tinnefeld Enrichment of cross-linked peptides from complex samples by charge-based fractional diagonal chromatography 
Waldemar Hoffmann Gas-Phase Infrared Spectroscopy of Ion Mobility-Selected Amyloid Intermediates 
Melanie Göth Gas-Phase Microsolvation of Ubiquitin: Identification of Crown Ether Complexation Sites 
Patrick Knight Characterising the interaction of ataxin-3 and the poly-glutamine aggregation inhibitor QBP1 
Rasmus U. Jakobsen Implementation of online electrochemical reduction of proteins into a Hydrogen/ Deuterium exchange Mass Spectrometry workflow 
Merkle P.S. Studying the Interaction between Affinity Enhanced T-Cell Receptors and Peptide-Bound Major Histocompatibility Complexes by Hydrogen/Deuterium Exchange Mass Spectrometry 
M. B. M. Nagel First full-length Thyroid stimulating Hormone-Receptor structure elucidated by low resolution techniques 
 Zsuzsanna Orban-Nemeth Comprehensive XL-MS reveals parallel orientation and versatile conformations of plant HOP2/MND1 
Gabriel Mazzucchelli De novo sequencing using MELD proteolysis coupled to "sequence assembly" algorithm  
M. Rosůlek From momomer to tetramer: Mass spectrometric study of protein WrbA multimerization 
Julia Lockhauserbäumer Norovirus infection pathway: glycan binding triggers structural changes
Johannes Heidemann Structural investigation of co-assembled clathrin adaptor protein complexes by native mass spectrometry 
Boris Krichel Quaternary structure of endolysin reveals how its activity is regulated by a secondary translation product
Gili Ben-Nissan Native mass spectrometry as a tool to discover structural impairment of Parkinson’s disease related proteins
Jan Commandeur Improving desolvation on q-tof instruments for native applications 
 Elisabetta Boeri Erba Characterisation of Rip-2 kinase autophosphorylation and oligomeric state by mass spectrometry and crystallography
S. Chatterjee IM-MS characterisation of protein therapeutics 
Josephine Abi-Ghanem Effect of the non-canonical G•U wobble base pair on the binding mechanism of RNA kissing complexes
 Natàlia Carulla SDS-PAGE analysis of Aβ oligomers is disserving research into Alzheimer´s disease: appealing for ESI-IM-MS
E.Grifnée, G. Mazzuchelli Strutural characterisation of protein by using enzymatic reactor
Tomas Jecmen Expression of photo-reactive cytochrome b 5  reductase for mapping its interaction surface with redox partner cytochrome b 5
R. Ptackova Photo cross-linking via L-Photo-Met and MS to study the interaction of 14-3-3 with phosphorylated Raf peptide 
Miroslav Šulc The photo-initiated cross-linking via pMet and MS: the tool to study the protein-protein interactions 
Moritz Fischer Synthesis of less hydrophobic Derivatives of MS/MS-Cleavable Cross-Linkers: Synthesis and Evaluation of Reactivity for effective Protein Structure Analysis 
Christoph Hage Dissociation behavior of bifunctional TEMPO-benzyl active ester for peptide structure analysis by free radical initiated peptide sequencing (FRIPS) mass spectrometry in negative ion mode 
Rico Schwarz Monitoring Conformational Changes in Peroxisome Proliferator-Activated Receptor β/δ by Cross-Linking/Mass Spectrometry 
Christine Piotrowski Structural Analysis of the bMunc13-2/Calmodulin Interaction by Cross-Linking and Mass Spectrometry 
Fan Liu Proteome-wide profiling of the structure and interaction of protein assemblies by cross-linking mass spectrometry 
Björn Häupl Characterization of protein kinase D2 complexes by chemical cross-linking and mass spectrometry 
Konstanze Bosse SILAC-Based Secretome Analysis of Non-Small Cell Lung Cancer Cell Lines 
C. U. Schräder A comprehensive look at the cross-linking pattern of human, bovine and frog elastin 
J. Wang Production and characterization of elastin-like biomaterial in vitro cross-linked by lysyl oxidase like 2 protein
A.C. Mora Huertas Insights into the age-related changes in elastic fibers from skin 
Gudrun Rutsdottir, Katja Bernfur Characterization of V181A, a dimeric Hsp21 mutant by SAXS and CXMS
Michael Götze Efficient Identification of Cross-Linked Peptides with StavroX and MeroX in Structural Proteomics 
Lutz Fischer False Discovery Rate Estimation in Cross-Linking/Mass Spectrometry and application in Protein Structure Determination 
Möller, I.R. Applying Hydrogen-Deuterium Exchange Mass Spectrometry to Analyze the Dynamics and Drug Binding of the Human Serotonin Transporter 
Petr Halada Expanding the acid protease toolbox for H/D exchange
 Michael O. Glocker Ion Mobility and Surface Topology Mapping Reveals the Cause  of the Protein G´e - IgG Affinity Switch 
 Peter Højrup  An Integrated Approach for Probing Protein Structure by Mass Spectrometry
Petr Man Transcription factor – DNA interaction studied by structural mass spectrometry 
Alan Kadek Electrostatics-driven conformational dynamics of cellobiose dehydrogenase probed by structural mass spectrometry 
Maxime Bourguet Structural MS for the characterization of yeast U3 snoRNP assembly
Nicholas J. Bond Structural and molecular studies of IL-33 disulphide isoforms 
Sandra Richter Proteomic identification of reinforcement- polarity dependently regulated synaptic proteins in an auditory learning paradigm in mice 
Edwin De Pauw Bottom-up and top-down high-throughput sequencing of toxins with pharmacological interest 
Leonhard H. Urner Photoswitchable Nanocarriers for Gas Phase Membrane Protein Analysis 
Gerard Comamala Studying Back-exchange Effects on Protein Analysis by Hydrogen/Deuterium exchange Mass Spectrometry
Sabrina Pfennig Regulation of the Chromatin Remodeler ISWI by its N-terminal domain
Christian Arlt 3D-Structural Characterization of the N- and C-terminal Regions of p53 by Combining Cross-Linking/MS and Native MS 
Jiří Hausner  Dynamics of chloride channel from E. coli studied by hydrogen/deuterium exchange

Symposium location is the Leopoldina building, German Academy of Sciences, Jagerberg 1, 06108 Halle/Saale (photo is a courtesy of Markus Scholz, Halle).
Current program includes talks on Thursday from 9AM and Friday till 5PM and conference dinner on Thursday night.
Halle is easily accessible by train from Leipzig/Halle airport (LEJ, 10 minutes, EUR6), from Berlin-Tegel or Berlin-Schoenefeld airports (TXL, SXF, 1.5 hours, EUR19) and from Frankfurt airport (FRA, 4 hours, EUR29).
Some of the hotels in Halle: Hotel Dorint Charlottenhof Dorotheenstr. 12, 06108 Halle/Saale , Hotel ?Ankerhof? Ankerstr. 2 , 06108 Halle/Saale , Dormero Hotel ?Rotes Ross? Franckestr. 1, 06110 Halle/Saale
If you have any questions, please email us at symposium@structuralproteomics.net
Registration and abstract submissions are open now. Registration fee is EUR 135.00. Late registration fee of EUR 175.00 applies from October 15, 2015.
To pay registation fee with credit card please use "Registration" button below. On the payment page please add your name to the "Participant name" box if it is different from the name of cardholder.
If you cannot use credit cards please contact us for the invoce to pay registration fees to the symposium bank account in Halle.
For registration, abstracts and payments please contact Andrea Sinz at Andrea.sinz@pharmazie.uni-halle.de
To pay by credit card or PayPal account a contribution for the symposium support please select the contribution option from drop-down menu and click "Sponsorship" button. On the payment page please add the name of your company to the "Company Name:" box.

Sponsors:

www.proteincentre.com www.creativemolecules.com www.waters.com

www.msvision.eu www.thermoscientific.com www.shimadzu.com www.sciex.com

www.isotope.com www.jeol.com www.bcpn.ca

www.bruker.com www.mrmproteomics.com

 

Previous Symposia on Structural proteomics:

4SSP 2014 Antwerp, Belgium
3SSP 2013 Prague, Czech Republic
2SSP 2012 Vancouver, BC, Canada
1SSP 2011 Banff, AB, Canada