The Sinz lab is since 2007 the department of Pharmaceutical Chemistry and Bioanalytics as part of the Institute of Pharmacy of the Martin-Luther-University Halle-Wittenberg.
The focus of our laboratory is the development of novel tools and workflows to promote the crosslinking/mass spectrometry (XL-MS) approach. XL-MS has become immensely popular in the past years to study protein 3D-structures and to delineate protein-protein interactions.
Just recently, we have been focussing on the development and application of MS-cleavable cross-linkers. The difference between non-cleavable and cleavable cross-linkers is that the signal patterns in MS fragmentation give a unique signature to identify cross-links when MS-cleavable cross-linkers are employed. We just published a proteome wide XL-MS workflow using the MS-cleavable cross-linker , disuccinimidyl dibutyric urea (DBSU). DBSU contains a urea group that is cleaved by collision-induced dissociation (CID). Accompanying the novel workflow, we upgraded our in-house software tool MeroX 2.0 so it can be applied to conduct fully automated and reliable large-scale protein network analyses.